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  • visualized clear margins and small tumor implants

    2020-08-12

    visualized clear margins and small tumor implants in the present study with this probe.
    Conclusion
    Claudin-1 is a useful target for NIR antibody-based imaging for visualization of primary and metastatic colorectal tumors for future use in fluorescence-guided surgery.
    Acknowledgment
    Authors’ contributions: H.M.H. wrote the manuscript and was actively involved in each step of the experimental pro-cess. T.M.L. was involved in the initial testing of Claudin-1 antibody and imaging. S.A. was actively involved in each step of the experimental process and contributed to the ma-terials and methods portion of the article. F.F. was involved in much of the preparation and execution of experiments. S.K.B. was involved in preliminary claudin research and in contri-bution of anti-Claudin antibody. R.M.H. was involved in revi-sion and editing of the article. P.D. contributed to information of claudin and provided anti-Claudin-1 antibody for initial experiments. She was also involved in editing the manuscript and oversight of the research design. M.B. was involved in oversight of the research design, implementation, and editing of the manuscript.
    Disclosure
    R.M.H. is an unsalaried affiliate of AntiCancer, Inc that offers PDOX models for contract research. The other authors report no proprietary or commercial interest in any product mentioned or concept discussed in this article.
    3. Maawy AA, Hiroshima Y, Zhang Y, et al. Near infra-red photoimmunotherapy with anti-CEA-IR700 results in extensive tumor lysis and a significant decrease in tumor burden in orthotopic mouse models of pancreatic cancer. PLoS One. 2015;10:e0121989.
    4. Hiroshima Y, Maawy A, Zhang Y, et al. Photoimmunotherapy inhibits tumor recurrence after surgical resection on a pancreatic cancer patient-derived orthotopic xenograft (PDOX) nude mouse model. Ann Surg Oncol. 2015;22(Suppl 3):S1469eS1474.
    5. Lwin TM, Miyake K, Murakami T, et al. Fluorescent humanized anti-CEA antibody specifically labels metastatic pancreatic cancer in a patient-derived orthotopic xenograft (PDOX) mouse model. Oncotarget. 2018;9:37333e37342.
    6. Pope JL, Ahmad R, Bhat AA, Washington MK, Singh AB, Dhawan P. Claudin-1 overexpression in intestinal epithelial ABT 263 enhances susceptibility to adenamatous polyposis coli-mediated colon tumorigenesis. Mol Cancer. 2014;13:167.
    9. Pope JL, Bhat AA, Sharma A, et al. Claudin-1 regulates intestinal epithelial homeostasis through the modulation of Notch-signalling. Gut. 2014;63:622e634.
    10. Rabinsky EF, Joshi BP, Pant A, et al. Overexpressed claudin-1 can be visualized endoscopically in colonic adenomas in vivo. Cell Mol Gastroenterol Hepatol. 2016;2:222e237.
    11. Hoffman RM. Patient-derived orthotopic xenografts: better mimic of metastasis than subcutaneous xenografts. Nat Rev Cancer. 2015;15:451e452.
    13. van Keulen S, Nishio N, Fakurnejad S, et al. The clinical application of fluorescence-guided surgery in head and neck cancer. J Nucl Med. 2019.
    14. Boogerd LSF, Hoogstins CES, Schaap DP, et al. Safety and effectiveness of SGM-101, a fluorescent antibody targeting carcinoembryonic antigen, for intraoperative detection of colorectal cancer: a dose-escalation pilot study. Lancet Gastroenterol Hepatol. 2018;3:181e191.
    Contents lists available at ScienceDirect
    Polymer
    journal homepage: www.elsevier.com/locate/polymer
    Anti-colon cancer effect of matrix protein gene therapy with nanoparticles T
    Bilan Wanga,b, Daoke Yangc, Yangmei Shenb,d,∗ a Department of Pharmacy, West China Second University Hospital, Sichuan University, Chengdu, 610041, PR China
    b Key Laboratory of Birth Defects and Related Diseases of Women and Children, Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu, 610041, PR China c Tumor Hospital of First Affiliated Hospital of Zhengzhou University, Zhengzhou 450052, PR China
    d Department of Pathology, West China Second University Hospital, Sichuan University, Chengdu, 610041, PR China
    • PEG-PCL-PEG and DOTAP could assembly into nanomicelles.
    • MP/DPPP could inhibited cancer proliferation in vitro.
    • MP/DPPP could induced cancer apoptosis in vitro.
    • MP/DPPP could inhibit cancer growth in vivo.
    Keywords:
    Matrix protein
    Cancer
    PEG-PCL-PEG 
    The matrix protein (MP) is the core structure of the oncolytic vesicular stomatitis virus (VSV) and it played a vital role during the process of VSV infection and corresponding cytopathic effects in cancer treatment. In this study, a novel delivery synthesized by PEG-PCL-PEG and DOTAP (PPPD) was applied to encapsulate MP plas-mids to derive the advantage and overcome the drawback of VSV platform in the treatment of colon cancer. The MP/DPPP micelles were stable in physiological solution, with an average diameter of 102 nm and zeta potential of 5.4 mV. This delivery system has a high encapsulation rate of 96% and transfection efficiency of 36%. A prominent anti-cancer activity of MP/DPPP was found both in Ct26 cells and animal models. The mechanisms of antitumor were further explored in this research. Our results indicated that MP/DPPP micelles were promising agents for future clinical application in the treatment of colon cancer.